A nanobody?horseradish peroxidase fusion protein?based competitive ELISA for rapid detection of antibodies against porcine circovirus type 2
نویسندگان
چکیده
Abstract Background The widespread popularity of porcine circovirus type 2(PCV2) has seriously affected the healthy development pig industry and caused huge economic losses worldwide. A rapid reliable method is required for epidemiological investigation evaluating effect immunization. However, current methods PCV2 antibody detection are time-consuming or very expensive rarely meet requirements clinical application. we have constructed platform expressing nanobody(Nb)?horseradish peroxidase(HRP) fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus(NDV), previously. In present work, Nb-HRP protein-based competitive ELISA(cELISA) simple was developed using this anti-PCV2 in serum. Results Using phage display technology, 19 anti-PCV2-Cap nanobodies were screened from a PCV2-Cap immunized Bactrian camel . With platform, PCV2-Nb15?HRP then produced used sensitive reagent developing cELISA anti?PCV2 antibodies. cut?off value 20.72 %. Three hundreds sixty serum samples tested by both newly commercial kits. sensitivity specificity 99.68 % 95.92 %, respectively. coincidence rate two 99.17 When detecting 620 samples, good consistent (kappa = 0.954) found between results those Conclusions brief, based rapid, low-cost, useful nanobody-based tool serological evaluation vaccine efficacy indirect diagnosis infection.
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ژورنال
عنوان ژورنال: Journal of Nanobiotechnology
سال: 2021
ISSN: ['1477-3155']
DOI: https://doi.org/10.1186/s12951-021-00778-8